Journal article
Establishment and characterization of irinotecan-resistant human non-small cell lung cancer A549 cells
Molecular medicine reports, Vol.3(6), pp.1031-1034
11/01/2010
DOI: 10.3892/mmr.2010.366
PMID: 21472350
Abstract
Irinotecan (CTP-11) is a topoisomerase 1 inhibitor used in the treatment of colorectal cancer and non-small cell lung cancer (NSCLC). Despite an initial response to therapy, resistance to irinotecan reduces its efficacy. We isolated irinotecan-resistant human NSCLC A549 cells, termed A549/CTP-11R cells. A549/CTP-11R cells were resistant to irinotecan, as well as paclitaxel, gemcitabine and carboplatin. Curcumin, a nuclear factor-kappa B (NF-kappa B) inhibitor, increased the sensitivity to irinotecan of A549/CTP-11R cells. The expression level of Bcl-X-L and X-linked inhibitor of apoptosis protein, target genes of NF-kappa B, in A549/CTP-11R cells was higher than that in A549 cells. Our result suggests that the addition of curcumin to irinotecan reverses irinotecan resistance in NSCLC.
Details
- Title: Subtitle
- Establishment and characterization of irinotecan-resistant human non-small cell lung cancer A549 cells
- Creators
- Ryuji Ikeda - Kagoshima UniversityLee C. Vermeulen - Univ Wisconsin Hosp & Clin, Ctr Drug Policy, Madison, WI 53792 USAElim Lau - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAZhisheng Jiang - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAMarcia Pomplun - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAJill M. Kolesar - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USA
- Resource Type
- Journal article
- Publication Details
- Molecular medicine reports, Vol.3(6), pp.1031-1034
- Publisher
- Spandidos Publ Ltd
- DOI
- 10.3892/mmr.2010.366
- PMID
- 21472350
- ISSN
- 1791-2997
- eISSN
- 1791-3004
- Number of pages
- 4
- Language
- English
- Date published
- 11/01/2010
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984695791102771
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