Journal article
F-18-FDG Kinetics Parameters Depend on the Mechanism of Injury in Early Experimental Acute Respiratory Distress Syndrome
The Journal of nuclear medicine (1978), Vol.55(11), pp.1871-1877
11/01/2014
DOI: 10.2967/jnumed.114.140962
PMCID: PMC4258066
PMID: 25286924
Abstract
PET with F-18-FDG allows for noninvasive assessment of regional lung metabolism reflective of neutrophilic inflammation. This study aimed at determining during early acute lung injury whether local F-18-FDG phosphorylation rate and volume of distribution were sensitive to the initial regional inflammatory response and whether they depended on the mechanism of injury: endotoxemia and surfactant depletion. Methods: Twelve sheep underwent homogeneous unilateral surfactant depletion (alveolar lavage) and were mechanically ventilated for 4 h (positive end-expiratory pressure, 10 cm H2O; plateau pressure, 30 cm H2O) while receiving intravenous endotoxin (lipopolysaccharide-positive [LPS+] group; n = 6) or not (lipopolysaccharide-negative group; n = 6). F-18-FDG PET emission scans were then acquired. F-18-FDG phosphorylation rate and distribution volume were calculated with a 4-compartment model. Lung tissue expression of inflammatory cytokines was measured using real-time quantitative reverse transcription polymerase chain reaction. Results: F-18-FDG uptake increased in LPS+ (P = 0.012) and in surfactant-depleted sheep (P < 0.001). These increases were topographically heterogeneous, predominantly in dependent lung regions, and without interaction between alveolar lavage and LPS. The increase of F-18-FDG uptake in the LPS+ group was related both to increases in the F-18-FDG phosphorylation rate (P < 0.05) and to distribution volume (P < 0.01). F-18-FDG distribution volume increased with infiltrating neutrophils (P < 0.001) and phosphorylation rate with the regional expression of IL-1 beta (P = 0.026), IL-8 (P = 0.011), and IL-10 (P = 0.023). Conclusion: Noninvasive F-18-FDG PET-derived parameters represent histologic and gene expression markers of early lung injury. Pulmonary metabolism assessed with F-18-FDG PET depends on the mechanism of injury and appears to be additive for endotoxemia and surfactant depletion. F-18-FDG PET may be a valuable imaging biomarker of early lung injury.
Details
- Title: Subtitle
- F-18-FDG Kinetics Parameters Depend on the Mechanism of Injury in Early Experimental Acute Respiratory Distress Syndrome
- Creators
- Nicolas de Prost - Harvard UniversityYan Feng - Harvard UniversityTyler Wellman - Massachusetts General HospitalMauro R. Tucci - Harvard UniversityEduardo L. Costa - Massachusetts General HospitalGuido Musch - Harvard UniversityTilo Winkler - Harvard UniversityR. Scott Harris - Harvard UniversityJose G. Venegas - Harvard UniversityWei Chao - Massachusetts General HospitalMarcos F. Vidal Melo - Harvard University
- Resource Type
- Journal article
- Publication Details
- The Journal of nuclear medicine (1978), Vol.55(11), pp.1871-1877
- DOI
- 10.2967/jnumed.114.140962
- PMID
- 25286924
- PMCID
- PMC4258066
- NLM abbreviation
- J Nucl Med
- ISSN
- 0161-5505
- eISSN
- 1535-5667
- Publisher
- Soc Nuclear Medicine Inc
- Number of pages
- 7
- Grant note
- R01HL121228 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) HL 5R01HL086827; 1R01HL121228 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 11/01/2014
- Academic Unit
- Anesthesia
- Record Identifier
- 9985141865902771
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