Journal article
Induction of Endogenous Uncoupling Protein 3 Suppresses Mitochondrial Oxidant Emission during Fatty Acid-supported Respiration
The Journal of biological chemistry, Vol.282(43), pp.31257-31266
10/26/2007
DOI: 10.1074/jbc.M706129200
PMID: 17761668
Abstract
Uncoupling protein 3 (UCP3) expression increases dramatically in skeletal muscle under metabolic states associated with elevated lipid metabolism, yet the function of UCP3 in a physiological context remains controversial. Here, in situ mitochondrial H2O2 emission and respiration were measured in permeabilized fiber bundles prepared from both rat and mouse (wild-type) gastrocnemius muscle after a single bout of exercise plus 18 h of recovery (Ex/R) that induced a ∼2–4-fold increase in UCP3 protein. Elevated uncoupling activity (i.e. GDP inhibitable) was evident in Ex/R fibers only upon the addition of palmitate (known activator of UCP3) or under substrate conditions eliciting substantial rates of H2O2 production (i.e. respiration supported by succinate or palmitoyl-l-carnitine/malate but not pyruvate/malate), indicative of UCP3 activation by endogenous reactive oxygen species. In mice completely lacking UCP3 (ucp3–/–), Ex/R failed to induce uncoupling activity. Surprisingly, when UCP3 activity was inhibited by GDP (rats) or in the absence of UCP3 (ucp3–/–), H2O2 emission was significantly (p < 0.05) higher in Ex/R versus non-exercised control fibers. Collectively, these findings demonstrate that the oxidant emitting potential of mitochondria is increased in skeletal muscle during recovery from exercise, possibly as a consequence of prolonged reliance on lipid metabolism and/or altered mitochondrial biochemistry/morphology and that induction of UCP3 in vivo mediates an increase in uncoupling activity that restores mitochondrial H2O2 emission to non-exercised, control levels.
Details
- Title: Subtitle
- Induction of Endogenous Uncoupling Protein 3 Suppresses Mitochondrial Oxidant Emission during Fatty Acid-supported Respiration
- Creators
- Ethan J Anderson - John B. Pierce Laboratory and Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519Hanae Yamazaki - John B. Pierce Laboratory and Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519P. Darrell Neufer - John B. Pierce Laboratory and Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.282(43), pp.31257-31266
- DOI
- 10.1074/jbc.M706129200
- PMID
- 17761668
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 10/26/2007
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Fraternal Order of Eagles Diabetes Research Center; Health, Sport, and Human Physiology
- Record Identifier
- 9984065694802771
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