Journal article
Isolation and characterization of gemcitabine-resistant human non-small cell lung cancer A549 cells
International journal of oncology, Vol.38(2), pp.513-519
02/01/2011
DOI: 10.3892/ijo.2010.866
PMID: 21152861
Abstract
Gemcitabine is an effective chemotherapy against non-small cell lung cancer (NSCLC). However, resistance to gemcitabine reduces its efficacy. We have isolated gemcitabine-resistant human non-small cell lung cancer A549 cells, termed A549/GR cells. A549/GR cells were resistant to gemcitabine as well as paclitaxel and docetaxel but not carboplatin and irinotecan. The expression level of multidrug resistance protein 7 (MRP7) in A549/GR cells was higher than that in A549 cells, and the inhibitor of MRP7 by cepharanthine increased the sensitivity to gemcitabine in A549/GR cells. These findings indicate that cepharanthine reversed gemcitabine resistance. To determine predictive molecular markers of gemcitabine resistance for more effective treatment of these tumors, we performed PCR array. We identified that CDKN1A/p21, CYP3A5, microsomal epoxide hyrolase 1 (EPHX1) and ABCC6 (MRP6) were up-regulated >5-fold in A549/GR cells. Gemcitabine also induced the expression of p21 and CYP3A5 in A549 cells. A better understanding of the characterization and mechanism of the resistance to gemcitabine in A5491GR cells may help identify agents that reverse clinical gemcitabine resistance in NSCLC.
Details
- Title: Subtitle
- Isolation and characterization of gemcitabine-resistant human non-small cell lung cancer A549 cells
- Creators
- Ryuji Ikeda - Kagoshima UniversityLee C. Vermeulen - Univ Wisconsin, Sch Pharm, Div Pharm Practice, Madison, WI 53705 USAElim Lau - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAZhisheng Jiang - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAKamakshi Sachidanandam - Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53705 USAKatsushi Yamada - Kagoshima UniversityJill M. Kolesar - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- International journal of oncology, Vol.38(2), pp.513-519
- Publisher
- Spandidos Publ Ltd
- DOI
- 10.3892/ijo.2010.866
- PMID
- 21152861
- ISSN
- 1019-6439
- eISSN
- 1791-2423
- Number of pages
- 7
- Language
- English
- Date published
- 02/01/2011
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984696554402771
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