Micro-Autoradiographic Assessment of Cell Types Contributing to 2-Deoxy-2-[F-18]Fluoro-d-Glucose Uptake During Ventilator-Induced and Endotoxemic Lung Injury

Dalia Saha; Kazue Takahashi; Nicolas de Prost; Tilo Winkler; Miguel Pinilla-Vera; Rebecca M. Baron; Marcos F. Vidal Melo

doi:10.1007/s11307-012-0575-x

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Micro-Autoradiographic Assessment of Cell Types Contributing to 2-Deoxy-2-[F-18]Fluoro-d-Glucose Uptake During Ventilator-Induced and Endotoxemic Lung Injury

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Micro-Autoradiographic Assessment of Cell Types Contributing to 2-Deoxy-2-[F-18]Fluoro-d-Glucose Uptake During Ventilator-Induced and Endotoxemic Lung Injury

Dalia Saha, Kazue Takahashi, Nicolas de Prost, Tilo Winkler, Miguel Pinilla-Vera, Rebecca M. Baron and Marcos F. Vidal Melo

Molecular imaging and biology, Vol.15(1), pp.19-27

02/01/2013

DOI: 10.1007/s11307-012-0575-x

PMCID: PMC6391052

PMID: 22752654

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https://www.ncbi.nlm.nih.gov/pmc/articles/6391052View

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Abstract

The aim of the study was to use micro-autoradiography to investigate the lung cell types responsible for 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) uptake in murine models of acute lung injury (ALI). C57/BL6 mice were studied in three groups: controls, ventilator-induced lung injury (VILI), and endotoxin. VILI was produced by high tidal volumes and zero end-expiratory pressure and endotoxin ALI, by intranasal administration. Following FDG injection, the lungs were processed and exposed to autoradiographic emulsion. Grain density over cells was used to quantify FDG uptake. Neutrophils, macrophages, and type 2 epithelial cells presented higher grain densities during VILI and endotoxin ALI than controls. Remarkably, cell grain density in specific cell types was dependent on the injury mechanism. Whereas macrophages showed high grain densities during endotoxin ALI, similar to those exhibited by neutrophils, type 2 epithelial cells demonstrated the second highest grain density (with neutrophils as the highest) during VILI. In murine models of VILI and endotoxin ALI, FDG uptake occurs not only in neutrophils but also in macrophages and type 2 epithelial cells. FDG uptake by individual cell types depends on the mechanism underlying ALI.

Life Sciences & Biomedicine

Radiology, Nuclear Medicine & Medical Imaging

Science & Technology

Details

Title: Subtitle: Micro-Autoradiographic Assessment of Cell Types Contributing to 2-Deoxy-2-[F-18]Fluoro-d-Glucose Uptake During Ventilator-Induced and Endotoxemic Lung Injury
Creators: Dalia Saha - Harvard University
Kazue Takahashi - Harvard University
Nicolas de Prost - Harvard University
Tilo Winkler - Massachusetts General Hospital
Miguel Pinilla-Vera - Harvard University
Rebecca M. Baron - Brigham and Women's Hospital
Marcos F. Vidal Melo - Massachusetts General Hospital
Resource Type: Journal article
Publication Details: Molecular imaging and biology, Vol.15(1), pp.19-27
DOI: 10.1007/s11307-012-0575-x
PMID: 22752654
PMCID: PMC6391052
NLM abbreviation: Mol Imaging Biol
ISSN: 1536-1632
eISSN: 1860-2002
Publisher: Springer Nature
Number of pages: 9
Grant note: HL 5R01HL086827 / National Heart, Lung, and Blood Institute/National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
Language: English
Date published: 02/01/2013
Academic Unit: Anesthesia
Record Identifier: 9985142948402771

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