Journal article
Monoamine Oxidase is a Major Determinant of Redox Balance in Human Atrial Myocardium and is Associated With Postoperative Atrial Fibrillation
Journal of the American Heart Association, Vol.3(1), pp.e000713-n/a
02/2014
DOI: 10.1161/JAHA.113.000713
PMCID: PMC3959694
PMID: 24572256
Abstract
Background
Onset of postoperative atrial fibrillation (POAF) is a common and costly complication of heart surgery despite major improvements in surgical technique and quality of patient care. The etiology of POAF, and the ability of clinicians to identify and therapeutically target high‐risk patients, remains elusive.
Methods and Results
Myocardial tissue dissected from right atrial appendage (RAA) was obtained from 244 patients undergoing cardiac surgery. Reactive oxygen species (ROS) generation from multiple sources was assessed in this tissue, along with total glutathione (GSHt) and its related enzymes GSH‐peroxidase (GPx) and GSH‐reductase (GR). Monoamine oxidase (MAO) and NADPH oxidase were observed to generate ROS at rates 10‐fold greater than intact, coupled mitochondria. POAF risk was significantly associated with MAO activity (Quartile 1 [Q1]: adjusted relative risk [ARR]=1.0; Q2: ARR=1.8, 95% confidence interval [CI]=0.84 to 4.0; Q3: ARR=2.1, 95% CI=0.99 to 4.3; Q4: ARR=3.8, 95% CI=1.9 to 7.5; adjusted Ptrend=0.009). In contrast, myocardial GSHt was inversely associated with POAF (Quartile 1 [Q1]: adjusted relative risk [ARR]=1.0; Q2: ARR=0.93, 95% confidence interval [CI]=0.60 to 1.4; Q3: ARR=0.62, 95% CI=0.36 to 1.1; Q4: ARR=0.56, 95% CI=0.34 to 0.93; adjusted Ptrend=0.014). GPx also was significantly associated with POAF; however, a linear trend for risk was not observed across increasing levels of the enzyme. GR was not associated with POAF risk.
Conclusions
Our results show that MAO is an important determinant of redox balance in human atrial myocardium, and that this enzyme, in addition to GSHt and GPx, is associated with an increased risk for POAF. Further investigation is needed to validate MAO as a predictive biomarker for POAF, and to explore this enzyme's potential role in arrhythmogenesis.
Details
- Title: Subtitle
- Monoamine Oxidase is a Major Determinant of Redox Balance in Human Atrial Myocardium and is Associated With Postoperative Atrial Fibrillation
- Creators
- Ethan J Anderson - East Carolina UniversityJimmy T Efird - East Carolina UniversityStephen W Davies - East Carolina UniversityWesley T O'Neal - Wake Forest University School of MedicineTimothy M Darden - East Carolina UniversityKathleen A Thayne - East Carolina UniversityLalage A Katunga - East Carolina UniversityLinda C Kindell - East Carolina UniversityT. Bruce Ferguson - East Carolina UniversityCurtis A Anderson - East Carolina UniversityW. Randolph Chitwood - East Carolina UniversityTheodore C Koutlas - East Carolina UniversityJ. Mark Williams - East Carolina UniversityEvelio Rodriguez - Saint Thomas Heart Cardiovascular SurgeryAlan P Kypson - East Carolina University
- Resource Type
- Journal article
- Publication Details
- Journal of the American Heart Association, Vol.3(1), pp.e000713-n/a
- DOI
- 10.1161/JAHA.113.000713
- PMID
- 24572256
- PMCID
- PMC3959694
- ISSN
- 2047-9980
- eISSN
- 2047-9980
- Number of pages
- 12
- Grant note
- National Institutes of Health (R21HL098780)
- Language
- English
- Date published
- 02/2014
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Fraternal Order of Eagles Diabetes Research Center; Health and Human Physiology
- Record Identifier
- 9984065316302771
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