Journal article
NAD(P)H: quinone oxidoreductase enhances proliferation inhibition by 4-hydroxytamoxifen
Anticancer research, Vol.22(3), pp.1475-1480
05/01/2002
PMID: 12168826
Abstract
The nonquinone compound, 4-OHT, was identified as a NQOR substrate as measured by reduction in the M7T assay. 4-OHT was then evaluated for effects on proliferation inhibition in two independent cell model systems, the MCF7 (NQO1+), MCF7 + dicoumarol (NQO1-) and the CHO (NQO1-) CHO-812 (NQO1+). NQOR activity predicts inhibition of proliferation by 4-OHT in these two models MCF7s were more sensitive to 4-OH tamoxifen with a PI50 of 2.65 nM than MCF-7 + dicoumarol with a PI50 of 1.84 microM (p<0.05, by one way ANOVA). CHO812s were also more sensitive to proliferation inhibition by 4-OH tamoxifen with a PI50 of 80.2 microM compared to the CHOs with a PI50 of 93.1 microM (p<0.05, by one-way ANOVA). Suggesting: 1) 4-OH tamoxifen is reduced by NQOR and 2) the reduced product is more active than the parent compound.
Details
- Title: Subtitle
- NAD(P)H: quinone oxidoreductase enhances proliferation inhibition by 4-hydroxytamoxifen
- Creators
- Peter G Allen - University of Wisconsin–MadisonJill M Kolesar
- Resource Type
- Journal article
- Publication Details
- Anticancer research, Vol.22(3), pp.1475-1480
- PMID
- 12168826
- ISSN
- 0250-7005
- eISSN
- 1791-7530
- Language
- English
- Date published
- 05/01/2002
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984695803102771
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