Journal article
Quantitative analysis of NQO1 gene expression by RT-PCR and CE-LIF
JOURNAL OF CAPILLARY ELECTROPHORESIS, Vol.2(6), pp.287-290
11/01/1995
PMID: 9384788
Abstract
A capillary electrophoresis-laser-induced fluorescence (CE-LIF) method to quantitate reverse transcription-polymerase chain reaction (RT-PCR) products of NAD(P)H:quinone acceptor oxidoreductase (NQO1) derived from whole blood alter amplification with a reaction-specific internal standard is reported. The internal standard eliminates variability within the PCR (Hoffman-La Roche, Inc., Nutley, NJ, U.S.A.), while analysis by CE-LIF adds sensitivity and reduces variability associated with isotopic detection. Both the PCR and CE aspects of the assay are precise, with migration time precision oi less than 1% and peak area ratio precisions of 9.8-15%. Future applications of this technique may include the analysis of gene therapy, oligonucleotides, and point mutations.
Details
- Title: Subtitle
- Quantitative analysis of NQO1 gene expression by RT-PCR and CE-LIF
- Creators
- J M KolesarJ D RizzoJ G Kuhn
- Resource Type
- Journal article
- Publication Details
- JOURNAL OF CAPILLARY ELECTROPHORESIS, Vol.2(6), pp.287-290
- Publisher
- I S C Technical Publications, Inc
- PMID
- 9384788
- ISSN
- 1079-5383
- Number of pages
- 4
- Language
- English
- Date published
- 11/01/1995
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984695796602771
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