Journal article
Three-Dimensional Quantification of Collagen Microstructure During Tensile Mechanical Loading of Skin
Frontiers in bioengineering and biotechnology, Vol.9, pp.642866-642866
03/03/2021
DOI: 10.3389/fbioe.2021.642866
PMCID: PMC7966723
PMID: 33748088
Abstract
Skin is a heterogeneous tissue that can undergo substantial structural and functional changes with age, disease, or following injury. Understanding how these changes impact the mechanical properties of skin requires three-dimensional (3D) quantification of the tissue microstructure and its kinematics. The goal of this study was to quantify these structure-function relationships
via
second harmonic generation (SHG) microscopy of mouse skin under tensile mechanical loading. Tissue deformation at the macro- and micro-scale was quantified, and a substantial decrease in tissue volume and a large Poisson’s ratio was detected with stretch, indicating the skin differs substantially from the hyperelastic material models historically used to explain its behavior. Additionally, the relative amount of measured strain did not significantly change between length scales, suggesting that the collagen fiber network is uniformly distributing applied strains. Analysis of undeformed collagen fiber organization and volume fraction revealed a length scale dependency for both metrics. 3D analysis of SHG volumes also showed that collagen fiber alignment increased in the direction of stretch, but fiber volume fraction did not change. Interestingly, 3D fiber kinematics was found to have a non-affine relationship with tissue deformation, and an affine transformation of the micro-scale fiber network overestimates the amount of fiber realignment. This result, along with the other outcomes, highlights the importance of accurate, scale-matched 3D experimental measurements when developing multi-scale models of skin mechanical function.
Details
- Title: Subtitle
- Three-Dimensional Quantification of Collagen Microstructure During Tensile Mechanical Loading of Skin
- Creators
- Alan E Woessner - University of Arkansas at FayettevilleJake D Jones - Department of Biomedical Engineering, University of ArkansasNathan J Witt - University of IowaEdward A Sander - University of Iowa, Roy J. Carver Department of Biomedical EngineeringKyle P Quinn - University of Arkansas at Fayetteville
- Resource Type
- Journal article
- Publication Details
- Frontiers in bioengineering and biotechnology, Vol.9, pp.642866-642866
- DOI
- 10.3389/fbioe.2021.642866
- PMID
- 33748088
- PMCID
- PMC7966723
- NLM abbreviation
- Front Bioeng Biotechnol
- ISSN
- 2296-4185
- eISSN
- 2296-4185
- Publisher
- Frontiers Media S.A
- Grant note
- National Institute on Aging National Institute of Biomedical Imaging and Bioengineering Arkansas Biosciences Institute
- Language
- English
- Date published
- 03/03/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Chemical and Biochemical Engineering
- Record Identifier
- 9984116622202771
Metrics
86 Record Views